李凯;商佳胤;张娜;田淑芬;黄建全;王丹;苏宏;王超霞;

• 1:天津市农业科学院果树研究所
• 2:天津农学院

摘要(Abstract)：

为研究天津产区玫瑰香型葡萄品质特性,采用顶空固相微萃取(HS-SPME)及气相色谱—质谱技术(GCMS),内标-标准曲线定量、气味活性值分析及果实香气轮廓分析等方法,分析了‘巨玫瑰’葡萄的果实香气。结果显示:天津产区‘巨玫瑰’葡萄果实中共检测出49种香气化合物,定量结果显示,以酯类、醛类、醇类和萜烯类为主,其中乙酸乙酯、反式-2-己烯-1-醛、1-己醇、里那醇、正己醛、反式-2-己烯-1-醇、β-蒎烯和2-苯乙醇是主要香气成分。气味活性值结果显示,‘巨玫瑰’葡萄果实香气主要由17种活性呈香成分贡献,气味活性值由高到低依次为反式-2-己烯-1-醛、正己醛、丁酸乙酯、里那醇、异丁酸乙酯、1-辛烯-3-醇、己酸乙酯、苯乙醛、2-甲基丁酸乙酯、乙酸异戊酯、乙酸乙酯、香叶醇、反式-2-己烯-1-醇、正庚醛、香茅醇、丙酸乙酯和β-蒎烯。香气轮廓简单直观地显示,‘巨玫瑰’果实香气主要由植物类、水果类、脂肪类和花卉类气味构成。

关键词(KeyWords)： ‘巨玫瑰’;顶空固相微萃取;香气成分;气味活性值;香气轮廓

Abstract:

Keywords:

基金项目(Foundation): 天津市林果现代农业产业技术体系(ITTFPRS2018005);; 国家现代农业(葡萄)产业技术体系建设专项(CARS-29);; 天津市科技计划项目(16ZXZYNC00160)

作者(Author): 李凯;商佳胤;张娜;田淑芬;黄建全;王丹;苏宏;王超霞;

Email:

DOI: 10.13320/j.cnki.jauh.2021.0009

参考文献(References)：

• [1]Selli S,Cabaroglu T,Canbas A,et al.Effect of skin contact on the aroma composition of the musts of Vitis vinifera L.cv.Muscat of Bornova and Narince grown in Turkey[J].Food Chemistry,2003,81(3):341-347.
• [2]Roubelakis-Angelakis K A.Grapevine molecular physiology and biotechnology[M].New York:Springer Verlag,2009:293-330.
• [3]李记明,宋长冰,贺普超.葡萄与葡萄酒芳香物质研究进展[J]．杨凌：西北农业大学学报,1998,26(5):105-109.
• [4]Yang C X,Wang Y J,Liang Z C,et al.Volatiles of grape berries evaluated at the germplasm level by headspace-SPME with GC-CM[J].Food Chemistry,2009,114(3):1106-1114.
• [5]Fenoll J,Manso A,Hellín P,et al.Changes in the aromatic composition of the Vitis vinifera grape Muscat Hamburg during ripening[J].Food Chemistry,2009,114(2):420-428.
• [6]Yang C X,Wang Y J,Wu B H,et al.Volatile compounds evolution of three table grapes with different flavor during and after maturation[J].Food Chemistry,2011,128(4):823-830.
• [7]Schwab W,Wuest M.Understanding the constitutive and induced biosynthesis of mono-and sesquiterpenes in grapes (vitis vinifera):a key to unlocking the biochemical secrets of unique grape aroma profiles[J].Journal of Agricultural and Food Chemistry,2015,63(49):10591-10603.
• [8]Cacho J,Moncayo L,Palma J C,et al.The impact of grape variety on the aromatic chemical composition of non-aromatic Peruvian pisco[J].Food Research International,2013,54:373-381.
• [9]FAN W,XU Y,JIANG W,et al.Identification and quantification of impact aroma compounds in 4 non floral Vitis vinifera varieties grapes[J].Journal of Food Science,2010,75:S81-S88.
• [10]赵悦,孙玉霞,孙庆扬,等.不同产地酿酒葡萄“赤霞珠”果实中挥发性香气物质差异性研究[J].北方园艺,2016,355(4):23-28.
• [11]周建梅,曹耀鹏,贾名波,等.山东不同产区巨峰葡萄香气物质分析[J].中外葡萄与葡萄酒,2013(2):16-20．
• [12]商敬敏,牟京霞,刘建民,等.GC-MS法分析不同产地酿酒葡萄的香气成分[J].食品与机械,2011,27(5):52-57.
• [13]张娜,路凤珍,温晓敏,等.7个葡萄品种在天津的引种表现[J].中国果树,2016(6):36-40.
• [14]Guth H.Quantitation and sensory studies of character impact odorants of different white wine varieties[J].Journal of Agricultural and Food Chemistry,1997,45(8):3027-3032.
• [15]Noble A C,Arnold R A,Buechsenstein J,et al.Modification of a standardized system of wine aroma terminology[J].American Journal of Enology and Viticulture,1987,38(2),143-146.
• [16]Mcmahon C,Scadding G K.Le Nez du Vin-a quick test of olfaction[J].Clinical Otolaryngology and Allied Sciences,1996,21(3):278-280.
• [17]Wu Y,Duan S,Zhao L,et al.Aroma characterization based on aromatic series analysis in table grapes[J].Scientific Reports,2016,6:31116.
• [18]张文文,吴玉森,陈毓谨,等.3种巨峰系葡萄的香气特征[J].上海交通大学学报:农业科学版,2018,36(5):51-59,66.
• [19]Pino J A,Mesa J.Contribution of volatile compounds to mango (Mangifera indica L.) aroma[J].Flavour and Fragrance Journal,2006,21(2):207-213.
• [20]Buttery R,SeifertR,Guadagni D,et al.Characterization of additional volatile components of tomato[J].Journal of Agricultural and Food Chemistry,1971,19(3):524-529.
• [21]Buttery R G,Turnbaugh J G,Ling L C.Contribution of volatiles to rice aroma[J].Journal of Agricultural and Food Chemistry,1988,36(5):1006-1009.
• [22]Qian M C,Wang Y.Seasonal variation of volatile composition and odor activity value of'Marion'(Rubus spp.hyb) and'Thornless Evergreen'(R.Laciniatus L.)blackberries[J].Journal of Food Science,2005,70(1):C13-C20.
• [23]YANG C,LUO L,ZHANG H,et al.Common aromaactive components of propolis from 23 regions of China[J].Journal of the Science of Food and Agriculture,2010,90(7):1268-1282.
• [24]Pino J A,Queris O.Analysis of volatile compounds of mango wine[J].Food Chemistry,2011,125(4):1141-1146.
• [25]Peinado R A,Moreno J,Bueno J E,et al.Comparative study of aromatic compounds in two young white wines subjected to pre-fermentative cryomaceration[J].Food Chemistry,2004,84(4):585-590.
• [26]Flath R A,Black D R,Guadagni D G,et al.Identification and organoleptic evaluation of compounds in delicious apple essence[J].Journal of Agricultural and Food Chemistry,1967,15(1):29-35.
• [27]文彦.形成葡萄酒香气特征的关键香气成分及其呈香机制研究[D].杨凌:西北农林科技大学,2013.
• [28]Tamura H,Fukuda Y,Padrayuttawat A.Characterization of citrus aroma quality by odor threshold values[C]//Biotechnology for Improved Foods and Flavor,Washington DC:American Chemical Society,1996:282-294.
• [29]Rosen A A,Ettinger R T S B.Relationship of river water odor to specific organic contaminants[J].Journal of Water Pollution Control Federation,1963,35(6):777-782.
• [30]Genovese A,Lamorte S A,GAMBUTI A,et al.Aroma of Aglianico and Uva di Troia grapes by aromatic series[J].Food Research International,2013,53(1):15-23.
• [31]Franco M,Peinado R A,Medina M,et al.Off-vine grape drying effect on volatile compounds and aromatic series in must from Pedro Ximénez grape variety[J].Journal of Agricultural and Food Chemistry,2004,52(12):3905-3910.
• [32]Acree T,Arn H.Flavornet and human odor space[J/OL].Datu Inc,2004[2020-3-31].http://www.flavornet.org.
• [33]Mahattanatawee K,Perez-cacho P R,Davenport T,et al.Comparison of three lychee cultivar odor profiles using gas chromatography-olfactometry and gas chromatography-sulfur detection[J].Journal of Agricultural and Food Chemistry,2007,55(5):1939-1944.
• [34]Giri A,Osako K,Ohshima T.Identification and characterisation of headspace volatiles of fish miso,a Japanese fish meat based fermented paste,with special emphasis on effect of fish species and meat washing[J].Food Chemistry,2010,120(2):621-631.
• [35]Peinado R A,Mauricio J C,Moreno J.Aromatic series in sherry wines with gluconic acid subjected to different biological aging conditions by Saccharomyces cerevisiae var.capensis[J].Food Chemistry,2006,94(2):232-239.
• [36]Ruiz M J,Zea L,Moyano L,et al.Aroma active compounds during the drying of grapes cv.Pedro Ximenez destined to the production of sweet Sherry wine[J].European Food Research and Technology,2010,230(3):429-435.
• [37]García-carpintero E G,Sánchez-palomo E,Gallego M A G,et al.Volatile and sensory characterization of red wines from cv.Moravia Agria minority grape variety cultivated in La Mancha region over five consecutive vintages[J].Food Research International,2011,44(5):1549-1560.
• [38]Song C Z,Liu M Y,Meng J F,et al.Influence of foliage-sprayed zinc sulfate on grape quality and wine aroma characteristics of Merlot[J].European Food Research and Technology,2016,242(4):609-623.
• [39]Gómez-míguez M J,Gómez-míguez M,Vicario I M,et al.Assessment of colour and aroma in white wines vinifications:Effects of grape maturity and soil type[J].Journal of Food Engineering,2007,79(3):758-764.
• [40]Culler e L,Escuder o A,Cacho J,et al.Gaschromatography-olfactometry and chemical quantitative study of the aroma of six premium quality spanish aged red wines[J].Journal of agricultural and food chemistry,2004,52(6):1653.
• [41]何明茜.葡萄香气物质在两类杂交群体中的遗传规律[D].武汉:华中农业大学,2013.
• [42]Barbera D,Avellone G,Filizzola F,et al.Determination of terpene alcohols in Sicilian Muscat wines by HS-SPME-GC-MS[J].Journal of Asian Natural Products Research,2013,27:541-547.
• [43]谭伟,唐晓萍,董志刚,等.4个四倍体玫瑰香味鲜食葡萄品种与其亲本果实香气成分分析[J].果树学报,2017,34(4):435-443.
• [44]Larsen M,Poll L,Olsen C E.Evaluation of the aroma composition of some strawberry (Fragaria ananassa Duch) cultivars by use of odour threshold values[J].Zeitschrift Fur Lebensmittel Untersuchung Und Forschung,1992,195(6):536-539.
• [45]Burattl S,Rizzolo A,Benedetti S,et al.Electronic nose to detect strawberry aroma changes during osmotic dehydration[J].Journal of Food Science,2006,71(4):E184-E189.
• [46]孟聚星,姜建福,张国海,等.我国育成的葡萄新品种系谱分析[J].果树学报,2017,34(4):393-409.
• [47]徐桂珍,陈景隆.玫瑰香葡萄的优良芽变-沈阳玫瑰[J].新农业,1982(15):19.
• [48]Oliveira J M,Faria M,SáF,et al.C6-alcohols as varietal markers for assessment of wine origin[J].Analytica Chimica Acta,2006,563(1):300-309.