郭康康;崔欢;张诚;张博;陈立功;刘聚祥;董世山;

• 1:河北农业大学动物医学院
• 2:农业部动物疫病病原生物学华北科学观测实验站
• 3:河北省兽医生物技术创新中心

摘要(Abstract)：

对1株H7N9亚型禽流感毒株A/environment/Hebei/621/2017(H7N9)简称EN621进行基因测序和序列分析,研究其遗传进化趋势和分子生物学特征。结果显示,该毒株血凝素(Hemagglutinin,HA)和神经氨酸酶(Neuraminidase,NA)分别与人源流感毒株的HA和NA属于同一进化支进化而来,EN621毒株HA蛋白的裂解位点存在多个连续碱性氨基酸,符合典型高致病性AIV特征;HA蛋白上有4个糖基化位点与人源流感毒株HA蛋白糖基化位点一致,且第226位氨基酸为Q,优先与禽类α-2,3唾液酸受体结合;NA蛋白上有6个潜在糖基化位点与人源流感毒株NA蛋白糖基化位点一致;NP蛋白第184氨基酸位点由A突变为K,对于AIV的复制和致病性有着密切联系。本研究提示EN621毒株存在感染人的潜在可能性,应加强H7N9亚型禽流感病毒的监测,建立相应的防范机制,保障公共安全。

关键词(KeyWords)： H7N9 AIV;分子特征分析;潜在糖基化位点;遗传进化分析

Abstract:

Keywords:

基金项目(Foundation): 河北省重点研发计划项目(20326622D);; 河北省现代农业产业技术体系蛋肉鸡产业创新团队项目(HBCT2018150210);; 河北省创新能力提升计划项目(20567668H)

作者(Author): 郭康康;崔欢;张诚;张博;陈立功;刘聚祥;董世山;

Email:

DOI: 10.13320/j.cnki.jauh.2021.0016

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